Mitsuhiro Itaya

Keio University

Areas of Expertise:
Genome Designing, Genome Engineering, Molecular BIology, Molecular Genetics, Synthetic Biology

Selected Publications:
1.Itaya, M. and Tsuge, K. Chapter 9: Towards bacterial Genome Reconstruction: Comparison of expression profiles of genes from different cellular DNA vehicles, plasmids and genomes. in “Escherichia coli and Bacillus subtilis: The Frontiers of Molecular Microbiology Revisited, pp297-317 (2012): ISBN: 978-81-308-0492-7 Editors: Yoshito Sadaie and Kouji Matsumoto.
2.Kaneko, S. and Itaya, M. Production of Multi-Purpose BAC Clones in the Novel Bacillus subtilis Based Host Systems. in “Bacterial Artificial Chromosomes” edited by Chatterjee, P. InTech open. pp.119-136 (2011).
3.Itaya, M. and Tsuge, K. Construction and manipulation of giant DNA by a genome vector. Methods Enzymol. 498, pp.427-447 (2011)
4.Itaya, M. “News & Views” Nature Biotechnology 28, (7) 687-689(2010)
5.Itaya, M and Kaneko, S. Integration of stable extra-cellular DNA released from Escherichia coli into the Bacillus subtilis genome vector by culture mix method. Nucleic Acids Res. 38(8) 2551–2557 (2010).
6.Itaya, M. “Recombinant genomes” in Systems Biology and Synthetic Biology, pp.155-194, Editors: Pengcheng Fu; Martin Latterich; Sven Panke, John Wiley & Brothers, Inc. (2009).
7.Itaya, M., et al., Bottom-up genome assembly using the Bacillus subtilis genome vector. Nature Methods, 5, 43-45 (2008)
8.Itaya, M., et al., Combining two genomes in one Cell: Stable cloning of the Synechosystis PCC6803 genome in the Bacillus subtilis 168 genome. Proc. Natl. Acad. Sci., USA, 102, 15971-15976 (2005).
9.Kaneko, S, Akioka, M., & Itaya, M. DNA shuttling between plasmid vectors and a genome vector: Systematic conversion and preservation of DNA libraries using the Bacillus subtilis genome (BGM) vector. J. Mol. Biol. 349: 1036-1044 (2005).
10.Tsuge, K., Matsui, K., & Itaya, M. One step assembly of multiple DNA fragments with designed order and orientation in Bacillus subtilis plasmid. Nucleic Acids Res. 31: No. 21, e-133 (2003).
11.Mizrahi, V,. N. R. Nossal and M. Itaya, "Enzymes from other sourced thar degrade RNA of RNADNA hybrids" in Ribonucleases H (eds. R. J Crouch and J. J. Toulme) Paris-INSERM. pp, 163-179 (1998).
12.Kunst, F., et al., The complete genome sequence of the Gram-positive bacterium Bacillus subtilis. Nature, 390, 249-256 (1997).
13. 11.Itaya, M. " Physical map of the Bacillus subtilis 168 chromosome". in "Bacterial Genomes: Physical Structure and Analysis" (eds. de Bruijin, F.J., Lupski, J.R., and Weinstock, G.) Chapman & Hall, pp.613-615 (1997).
14.Itaya, M. & Tanaka. T. Experimental surgery to create subgenomes of Bacillus subtilis 168. Proc. Natl. Acad. Sci., USA. 94, 5378-5382 (1997).
15.Itaya, M. An estimation of minimal genome size required for life. FEBS Lett. 362, 257-260 (1995).
16.Itaya, M. & Tanaka, T. Complete physical map of the Bacillus subtilis 168 chromosome constructed by a gene-directed mutagenesis method. J. Mol. Biol. 220, 631-648 (1991).
17.Itaya, M. Isolation and characterization of a second ribonuclease H (RNase HII) of Esherichia coli K12 encoded by the rnhB gene. Proc. Natl. Acad. Sci., USA. 87,8587-8591 (1990).