 Mitsuhiro Itaya
Keio University
Areas of Expertise:
Genome Designing, Genome Engineering, Molecular
BIology, Molecular Genetics, Synthetic Biology
Selected Publications:
1.Itaya, M. and Tsuge, K. Chapter 9: Towards bacterial Genome Reconstruction:
Comparison of expression profiles of genes from different cellular DNA vehicles,
plasmids and genomes. in “Escherichia coli and Bacillus subtilis: The Frontiers
of Molecular Microbiology Revisited, pp297-317 (2012): ISBN: 978-81-308-0492-7
Editors: Yoshito Sadaie and Kouji Matsumoto.
2.Kaneko, S. and Itaya, M. Production of Multi-Purpose BAC Clones
in the Novel Bacillus subtilis Based Host Systems. in “Bacterial Artificial
Chromosomes” edited by Chatterjee, P. InTech open. pp.119-136 (2011).
3.Itaya, M. and Tsuge, K. Construction and manipulation of giant
DNA by a genome vector. Methods Enzymol. 498, pp.427-447 (2011)
4.Itaya, M. “News & Views” Nature Biotechnology
28, (7) 687-689(2010)
5.Itaya, M and Kaneko, S. Integration of stable extra-cellular
DNA released from Escherichia coli into the Bacillus subtilis genome vector
by culture mix method. Nucleic Acids Res. 38(8) 2551–2557
(2010).
6.Itaya, M. “Recombinant genomes” in Systems Biology and Synthetic
Biology, pp.155-194, Editors: Pengcheng Fu; Martin Latterich; Sven Panke,
John Wiley & Brothers,
Inc. (2009).
7.Itaya, M., et al., Bottom-up genome assembly using the Bacillus
subtilis genome vector. Nature Methods, 5, 43-45 (2008)
8.Itaya, M., et al., Combining two genomes in one Cell: Stable
cloning of the Synechosystis PCC6803 genome in the Bacillus subtilis 168
genome. Proc. Natl. Acad. Sci., USA, 102, 15971-15976 (2005).
9.Kaneko, S, Akioka, M., & Itaya,
M. DNA shuttling between plasmid vectors and a genome vector: Systematic
conversion and preservation of DNA libraries using the Bacillus subtilis
genome (BGM) vector. J. Mol. Biol. 349: 1036-1044 (2005).
10.Tsuge, K., Matsui, K., & Itaya,
M. One step assembly of multiple DNA fragments with designed order and orientation
in Bacillus subtilis plasmid. Nucleic Acids Res. 31: No. 21, e-133 (2003).
11.Mizrahi, V,. N. R. Nossal and M. Itaya, "Enzymes
from other sourced thar degrade RNA of RNADNA hybrids" in Ribonucleases H (eds. R. J Crouch and J. J. Toulme) Paris-INSERM. pp, 163-179
(1998).
12.Kunst, F., et al., The complete genome sequence of the Gram-positive
bacterium Bacillus subtilis. Nature, 390, 249-256 (1997).
13. 11.Itaya, M. " Physical map of the Bacillus
subtilis 168 chromosome". in "Bacterial Genomes: Physical Structure and Analysis" (eds. de Bruijin, F.J., Lupski, J.R., and Weinstock, G.) Chapman & Hall, pp.613-615 (1997).
14.Itaya, M. & Tanaka. T. Experimental surgery
to create subgenomes of Bacillus subtilis 168. Proc. Natl. Acad. Sci., USA.
94, 5378-5382 (1997).
15.Itaya, M. An estimation of minimal genome size required for
life. FEBS Lett. 362, 257-260 (1995).
16.Itaya, M. & Tanaka, T. Complete physical map
of the Bacillus subtilis 168 chromosome constructed by a gene-directed mutagenesis
method. J. Mol. Biol. 220, 631-648 (1991).
17.Itaya, M. Isolation and characterization of a second ribonuclease
H (RNase HII) of Esherichia coli K12 encoded by the rnhB gene. Proc. Natl.
Acad. Sci., USA. 87,8587-8591 (1990). |
